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lexA [2019-08-01 14:10:08]
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lexA [2019-08-01 14:10:08]

transcriptional repressor of the SOS regulon
Locus
BSU_17850
Isoelectric point
5.16
Molecular weight
22.70 kDa
Protein length
205 aa Sequence Blast
Gene length
618 bp Sequence Blast
Function
regulation of DNA damage repair
Product
transcriptional repressor of the SOS regulon
Essential
no
Synonyms
dinR
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Information processing → Genetics → DNA repair/ recombination
  • Information processing → Genetics → DNA repair/ recombination → Other proteins
  • Information processing → Regulation of gene expression → Transcription factors and their control → Transcription factors/ other

    • Gene

    Coordinates
    1,917,639 → 1,918,256

    The protein

    Catalyzed reaction/ biological activity

  • Represses uvrB, bstG, tagC, recA genes and itself by binding to the 14 bp palindromic sequence 5'-CGAACNNNNGTTCG-3'. In the presence of single-stranded DNA, RecA interacts with LexA causing an autocatalytic cleavage which disrupts the DNA-binding part of LexA, leading to derepression of the SOS regulon and eventually DNA repair. (according to Swiss-Prot)

    • Protein family

  • peptidase S24 family (single member, according to UniProt)

    • Structure

  • 1JHH (from E. coli, S119A mutant, 33% identity, 52% similarity) PubMed

    • Additional information

  • information on binding sites can be found in the PRODORIC2 database

    • Expression and Regulation

    Operons

    Genes
    lexA
    Description
    PubMed

    Regulatory mechanism

  • LexA: repression, PubMed, in LexA regulon
  • RocR: autorepression, in RocR regulon

    • Regulation

  • induced upon DNA damage (LexA) PubMed

    • Additional information

  • A ncRNA is predicted between 'fosB' and 'lexA' PubMed
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    Biological materials

    Mutant

  • BKE17850 (ΔlexA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTTTTCGCACCTCAAAAC, downstream forward: _UP4_TAATCTATTGATCTATCAGA
  • BKK17850 (ΔlexA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTTTTCGCACCTCAAAAC, downstream forward: _UP4_TAATCTATTGATCTATCAGA

    • References

    Reviews

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    Original publications

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